SIM1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SIM1.
Immunogen
A synthetic peptide corresponding to internal region of human SIM1.
Sequence
C-SGDRYRTEQYQS
Host
Goat
Theoretical MW (kDa)
80
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SIM1 polyclonal antibody (Cat # PAB24599) (1 ug/mL) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SIM1
Entrez GeneID
6492Protein Accession#
NP_005059.2Gene Name
SIM1
Gene Alias
bHLHe14
Gene Description
single-minded homolog 1 (Drosophila)
Gene Ontology
HyperlinkGene Summary
SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome. [provided by RefSeq
Other Designations
single-minded homolog 1
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Interactome
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Disease
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