CHIT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
CHIT1 polyclonal antibody (Cat # PAB24571) (1 ug/mL) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CHIT1.
Immunogen
A synthetic peptide corresponding to amino acids 409-423 near C-terminus of human CHIT1.
Sequence
EHGPSPGQDTFCQGK
Host
Goat
Theoretical MW (kDa)
48
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CHIT1 polyclonal antibody (Cat # PAB24571) (1 ug/mL) staining of Daudi lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHIT1
Entrez GeneID
1118Protein Accession#
NP_003456.1Gene Name
CHIT1
Gene Alias
CHI3, CHIT, FLJ00314, MGC125322
Gene Description
chitinase 1 (chitotriosidase)
Omim ID
600031Gene Ontology
HyperlinkGene Summary
Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. [provided by RefSeq
Other Designations
chitotriosidase|plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase
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Interactome
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Pathway
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Disease
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