TBP polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TBP.
Immunogen
A synthetic peptide corresponding to amino acids 39-50 at internal region of human TBP.
Sequence
C-TPQPIQNTNSLS
Host
Goat
Theoretical MW (kDa)
40
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_003185.1; NP_001165556.1).
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TBP polyclonal antibody (Cat # PAB24569) (1 ug/mL) staining of HeLa nuclear (1) and cytosolic (2) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TBP
Entrez GeneID
6908Protein Accession#
NP_003185.1;NP_001165556.1Gene Name
TBP
Gene Alias
GTF2D, GTF2D1, MGC117320, MGC126054, MGC126055, SCA17, TFIID
Gene Description
TATA box binding protein
Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. [provided by RefSeq
Other Designations
OTTHUMP00000017703
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Interactome
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Pathway
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Disease
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