SFTPA1/SFTPA2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SFTPA1/SFTPA2.
Immunogen
A synthetic peptide corresponding to amino acids 102-115 at internal region of human SFTPA1/SFTPA2.
Sequence
C-HLDEELQATLHDFR
Host
Goat
Theoretical MW (kDa)
30
Reactivity
Human
Specificity
This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1
Form
Liquid
Purification
Antigen affinity purification
Recommend Usage
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SFTPA1/SFTPA2 polyclonal antibody (Cat # PAB24551) (0.01 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SFTPA1
Entrez GeneID
653509Protein Accession#
NP_005402.3 (Gene ID : 653509);NP_001087239.2 (Gene ID : 653509);NP_001158117.1 (Gene ID : 653509);NP_001158118.1 (Gene ID : 653509);NP_001092138.1 (Gene ID : 729238)Gene Name
SFTPA1
Gene Alias
COLEC4, FLJ51913, SFTP1, SP-A, SP-A1
Gene Description
surfactant protein A1
Gene Ontology
HyperlinkGene Summary
pulmonary-associated protein A1|surfactant
Other Designations
surfactant, pulmonary-associated protein A1|surfactant, pulmonary-associated protein A1A
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Gene Info — SFTPA2
Entrez GeneID
729238Protein Accession#
NP_005402.3 (Gene ID : 653509);NP_001087239.2 (Gene ID : 653509);NP_001158117.1 (Gene ID : 653509);NP_001158118.1 (Gene ID : 653509);NP_001092138.1 (Gene ID : 729238)Gene Name
SFTPA2
Gene Alias
COLEC5, MGC189761, SP-A2, SPAII
Gene Description
surfactant protein A2
Gene Ontology
HyperlinkGene Summary
This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication. [provided by RefSeq
Other Designations
OTTHUMP00000019932|surfactant, pulmonary-associated protein A2|surfactant, pulmonary-associated protein A2A
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Interactome
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Disease
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