SENP3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of SENP3 polyclonal antibody (Cat # PAB2416) in SAOS-2 cell lysate. SENP3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SENP3 polyclonal antibody (Cat # PAB2416) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. (RIGHT) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SENP3 polyclonal antibody (Cat # PAB2416) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SENP3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SENP3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SENP3 polyclonal antibody (Cat # PAB2416) in SAOS-2 cell lysate. SENP3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SENP3 polyclonal antibody (Cat # PAB2416) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. (RIGHT) Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SENP3 polyclonal antibody (Cat # PAB2416) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — SENP3
Entrez GeneID
26168Protein Accession#
NP_056485;Q9H4L4Gene Name
SENP3
Gene Alias
DKFZp586K0919, DKFZp762A152, SMT3IP1, SSP3
Gene Description
SUMO1/sentrin/SMT3 specific peptidase 3
Gene Ontology
HyperlinkGene Summary
The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP3, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM
Other Designations
SUMO1/sentrin/SMT3 specific protease 3|sentrin/SUMO-specific protease 3
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Interactome
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Disease
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Publication Reference
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SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
Hochstrasser M.
Cell 2001 Oct; 107(1):5.
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Involvement of PIAS1 in the sumoylation of tumor suppressor p53.
Kahyo T, Nishida T, Yasuda H.
Molecular Cell 2001 Sep; 8(3):713.
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SUMO, ubiquitin's mysterious cousin.
Muller S, Hoege C, Pyrowolakis G, Jentsch S.
Nature Reviews. Molecular Cell Biology 2001 Mar; 2(3):202.
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SP-RING for SUMO: new functions bloom for a ubiquitin-like protein.
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