RSHL3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant RSHL3.
Immunogen
Recombinant protein corresponding to amino acids of human RSHL3.
Sequence
DVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney with RSHL3 polyclonal antibody (Cat # PAB22777) shows strong cytoplasmic positivity in cells in tubules at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with RSHL3 polyclonal antibody (Cat # PAB22777) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli. -
Gene Info — RSHL3
Entrez GeneID
345895Protein Accession#
Q5TD94Gene Name
RSHL3
Gene Alias
FLJ37974, MGC126303, dJ412I7.1
Gene Description
radial spokehead-like 3
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that appears to be a component the radial spoke head, as determined by homology to similar proteins in the biflagellate alga Chlamydomonas reinhardtii and other ciliates. Radial spokes, which are regularly spaced along cilia, sperm, and flagella axonemes, consist of a thin 'stalk' and a bulbous 'head' that form a signal transduction scaffold between the central pair of microtubules and dynein. Mutations in this gene cause primary ciliary dyskinesia 1, a disease arising from dysmotility of motile cilia and sperm. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000017071
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