SMG7 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SMG7.
Immunogen
Recombinant protein corresponding to amino acids of human SMG7.
Sequence
RDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with SMG7 polyclonal antibody (Cat # PAB22273) at 1:250-1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human esophagus with SMG7 polyclonal antibody (Cat # PAB22273) shows strong cytoplasmic positivity in squamous epithelial cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with SMG7 polyclonal antibody (Cat # PAB22273) at 1-4 ug/mL dilution shows positivity in cytoplasm. -
Gene Info — SMG7
Entrez GeneID
9887Protein Accession#
Q92540Gene Name
SMG7
Gene Alias
C1orf16, EST1C, FLJ23717, KIAA0250, SGA56M, SMG-7
Gene Description
Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Omim ID
610964Gene Ontology
HyperlinkGene Summary
SMG7 is involved in nonsense-mediated mRNA decay (Fukuhara et al., 2005 [PubMed 15721257]).[supplied by OMIM
Other Designations
EST1 telomerase component homolog C|OTTHUMP00000033244|SMG-7 homolog|breast cancer-associated antigen SGA-56M|ever shorter telomeres 1C
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