DNMT3L polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HL-60 cell lysate with DNMT3L polyclonal antibody (Cat # PAB2218).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the DNMT3L polyclonal antibody (Cat # PAB2218) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DNMT3L.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DNMT3L.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HL-60 cell lysate with DNMT3L polyclonal antibody (Cat # PAB2218).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the DNMT3L polyclonal antibody (Cat # PAB2218) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — DNMT3L
Entrez GeneID
29947Protein Accession#
Q9UJW3Gene Name
DNMT3L
Gene Alias
MGC1090
Gene Description
DNA (cytosine-5-)-methyltransferase 3-like
Omim ID
606588Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq
Other Designations
cytosine-5-methyltransferase 3-like protein|human cytosine-5-methyltransferase 3-like protein
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Interactome
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Pathway
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Disease
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Publication Reference
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The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a.
Chedin F, Lieber MR, Hsieh CL.
PNAS 2002 Dec; 99(26):16916.
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The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a.
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