TMEM199 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TMEM199.
Immunogen
Recombinant protein corresponding to amino acids of human TMEM199.
Sequence
AGERLVRALGPGGELEPERLPRKLRAELEAALGKKHKGGDSSSGPQRLVSFRLIRDLHQHLRERDSKLYLHELLEGSEIYLPEVVKPPRNPELVARLEKIKIQLANEEYKRITRNVTCQDTRHGGTLSDLGKQVRSL
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:250-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with TMEM199 polyclonal antibody (Cat # PAB21999).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with TMEM199 polyclonal antibody (Cat # PAB21999) shows moderate cytoplasmic positivity in glandular cells. -
Gene Info — TMEM199
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Interactome
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Publication Reference
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Three unreported cases of TMEM199-CDG, a rare genetic liver disease with abnormal glycosylation.
Vajro P, Zielinska K, Ng BG, Maccarana M, Bengtson P, Poeta M, Mandato C, D'Acunto E, Freeze HH, Eklund EA.
Orphanet Journal of Rare Diseases 2018 Jan; 13(1):4.
Application:WB-Ce, Human, HepG2 cells, Human skin fibroblasts.
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Three unreported cases of TMEM199-CDG, a rare genetic liver disease with abnormal glycosylation.
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