TCF21 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TCF21 polyclonal antibody (Cat # PAB21931).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with TCF21 polyclonal antibody (Cat # PAB21931) shows moderate cytoplasmic and nuclear positivity in glandular cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant TCF21.
Immunogen
Recombinant protein corresponding to amino acids of human TCF21.
Sequence
LSDVEDLQEVEMLECDGLKMDSNKEFVTSNESTEESSNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQEGKQV
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:20-1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate).
Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TCF21 polyclonal antibody (Cat # PAB21931).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon with TCF21 polyclonal antibody (Cat # PAB21931) shows moderate cytoplasmic and nuclear positivity in glandular cells. -
Gene Info — TCF21
Entrez GeneID
6943Protein Accession#
O43680Gene Name
TCF21
Gene Alias
POD1, bHLHa23
Gene Description
transcription factor 21
Omim ID
603306Gene Ontology
HyperlinkGene Summary
TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017238|OTTHUMP00000017239|capsulin|epicardin|podocyte-expressed 1
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Interactome
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Disease
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