PRMT5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRMT5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRMT5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The PRMT5 polyclonal antibody (Cat # PAB2175) is used in Western blot to detect PRMT5 in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PRMT5
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Interactome
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Publication Reference
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mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
Pal S, Yun R, Datta A, Lacomis L, Erdjument-Bromage H, Kumar J, Tempst P, Sif S.
Molecular and Cellular Biology 2003 Nov; 23(21):7475.
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Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family.
Rho J, Choi S, Seong YR, Cho WK, Kim SH, Im DS.
The Journal of Biological Chemistry 2001 Jan; 276(14):11393.
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The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity.
Pollack BP, Kotenko SV, He W, Izotova LS, Barnoski BL, Pestka S.
The Journal of Biological Chemistry 1999 Oct; 274(44):31531.
Application:WB-Tr, Human, HeLa cells.
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mSin3A/histone deacetylase 2- and PRMT5-containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.
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