Myb (phospho T486) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of COS7 cell untreated (Lane 1) or treated with okadaic acid (Lane 2) by using Myb (phospho T486) polyclonal antibody (Cat # PAB19946) at 1:2500 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Myb.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T486 of mouse Myb.
Host
Rabbit
Theoretical MW (kDa)
75
Reactivity
Mouse
Specificity
Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
Form
Liquid
Purification
Affinity purification
Recommend Usage
ELISA (1:5000-1:16000)
Western Blot (1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of COS7 cell untreated (Lane 1) or treated with okadaic acid (Lane 2) by using Myb (phospho T486) polyclonal antibody (Cat # PAB19946) at 1:2500 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Myb
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