TRMT12 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRMT12.
Immunogen
A synthetic peptide corresponding to 19 amino acids near N-terminus of human TRMT12.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
TRMT12 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW2 are known to exist; this antibody will detect both isoforms.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (0.5 ug/mL)
Immunocytochemistry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TYW2 in K-562 cell lysate with TRMT12 polyclonal antibody (Cat # PAB19449) at 0.5 ug/mL.
Lane 1 : The absence of blocking peptide.
Lane 2 : The presence of blocking peptide.Immunocytochemistry
Immunocytochemical staining of K-562 cells with TRMT12 polyclonal antibody (Cat # PAB19449) at 10 ug/mL.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRMT12
Entrez GeneID
55039Protein Accession#
Q53H54Gene Name
TRMT12
Gene Alias
FLJ20772, TRM12, TYW2
Gene Description
tRNA methyltransferase 12 homolog (S. cerevisiae)
Omim ID
611244Gene Ontology
HyperlinkGene Summary
Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM
Other Designations
homolog of yeast tRNA methyltransferase|homolog of yeast tRNA methyltransferase 12|tRNA methyltranferase 12 homolog|tRNA-yW synthesizing protein 2
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