ECH1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HepG2 (Lane 1) and Jurkat (Lane 2) cell lysate with ECH1 polyclonal antibody (Cat # PAB18861) at 1 : 500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal kidney tissue showing cytoplasmic staining with ECH1 polyclonal antibody (Cat # PAB18861) at 1 : 100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant ECH1.
Immunogen
Recombinant protein corresponding to amino acids 145-325 of human ECH1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:10000-1:80000)
Western Blot (1:200-1:1000)
Immunohistochemistry (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 0.02% sodium azide
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 (Lane 1) and Jurkat (Lane 2) cell lysate with ECH1 polyclonal antibody (Cat # PAB18861) at 1 : 500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human fetal kidney tissue showing cytoplasmic staining with ECH1 polyclonal antibody (Cat # PAB18861) at 1 : 100 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ECH1
Entrez GeneID
1891Protein Accession#
NM_001398Gene Name
ECH1
Gene Alias
HPXEL
Gene Description
enoyl Coenzyme A hydratase 1, peroxisomal
Omim ID
600696Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq
Other Designations
delta3,5-delta2,4-dienoyl-CoA isomerase|dienoyl-CoA isomerase|peroxisomal enoyl-CoA hydratase 1|peroxisomal enoyl-coenzyme A hydratase-like protein
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Interactome
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Pathway
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