TNFSF14 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TNFSF14.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human TNFSF14.
Sequence
C-HEVNPAAHLTGANS
Host
Goat
Theoretical MW (kDa)
22
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TNFSF14 polyclonal antibody (Cat # PAB18727) (1 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TNFSF14
Entrez GeneID
8740Protein Accession#
NP_003798.2;NP_742011.1Gene Name
TNFSF14
Gene Alias
CD258, HVEML, LIGHT, LTg, TR2
Gene Description
tumor necrosis factor (ligand) superfamily, member 14
Omim ID
604520Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
delta transmembrane LIGHT|herpesvirus entry mediator A|ligand for herpesvirus entry mediator|tumor necrosis factor ligand superfamily, member 14|tumor necrosis factor receptor-like 2|tumor necrosis factor superfamily member LIGHT
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