GATA3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
GATA3 polyclonal antibody (Cat # PAB18658) (1.0 ug/mL) staining of Molt 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GATA3 polyclonal antibody (Cat # PAB18658) (3 ug/mL) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the germinal centre, B) staining of cells in the interfollicular area.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GATA3.
Immunogen
A synthetic peptide corresponding to amino acids at N-terminus of human GATA3.
Sequence
EVTADQPRWVSHH-C
Host
Goat
Theoretical MW (kDa)
50
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GATA3 polyclonal antibody (Cat # PAB18658) (1.0 ug/mL) staining of Molt 4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GATA3 polyclonal antibody (Cat # PAB18658) (3 ug/mL) staining of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) staining of cells in the germinal centre, B) staining of cells in the interfollicular area.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GATA3
Entrez GeneID
2625Protein Accession#
NP_001002295.1;NP_002042.1Gene Name
GATA3
Gene Alias
HDR, MGC2346, MGC5199, MGC5445
Gene Description
GATA binding protein 3
Gene Ontology
HyperlinkOther Designations
GATA-binding protein 3|OTTHUMP00000019070|OTTHUMP00000019071
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Interactome
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Disease
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