HSH2D polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HSH2D.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human HSH2D.
Sequence
C-HSHVGYTLSYKAQ
Host
Goat
Theoretical MW (kDa)
37
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
HSH2D polyclonal antibody (Cat # PAB18630) (0.3 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSH2D
Entrez GeneID
84941Protein Accession#
NP_116244.1Gene Name
HSH2D
Gene Alias
ALX, FLJ14886, HSH2
Gene Description
hematopoietic SH2 domain containing
Omim ID
608349Gene Ontology
HyperlinkGene Summary
T-cell activation requires 2 signals: recognition of antigen by the T-cell receptor (see TCR; MIM 186880) and a costimulatory signal provided primarily by CD28 (MIM 186760) in naive T cells. HSH2 is a target of both of these signaling pathways (Greene et al., 2003 [PubMed 12960172]).[supplied by OMIM
Other Designations
adaptor in lymphocytes of unknown function X|hematopoietic SH2 protein
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Interactome
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Disease
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