NR4A1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using NR4A1 polyclonal antibody (Cat # PAB18428).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using NR4A1 polyclonal antibody (Cat # PAB18428).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NR4A1.
Immunogen
A synthetic peptide corresponding to residues surrounding S351 of human NR4A1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to NR4A1.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, using NR4A1 polyclonal antibody (Cat # PAB18428).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using NR4A1 polyclonal antibody (Cat # PAB18428).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — NR4A1
Entrez GeneID
3164Protein Accession#
P22736Gene Name
NR4A1
Gene Alias
GFRP1, HMR, MGC9485, N10, NAK-1, NGFIB, NP10, NUR77, TR3
Gene Description
nuclear receptor subfamily 4, group A, member 1
Omim ID
139139Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
TR3 orphan receptor|early response protein NAK1|growth factor-inducible nuclear protein N10|hormone receptor|orphan nuclear receptor HMR|steroid receptor TR3
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Interactome
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Pathway
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Disease
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Publication Reference
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
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