NFATC4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using NFATC4 polyclonal antibody (Cat # PAB18192).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFATC4 polyclonal antibody (Cat # PAB18192).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NFATC4.
Immunogen
A synthetic peptide corresponding to residues surrounding S676 of human NFATC4.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to NFATC4.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells, using NFATC4 polyclonal antibody (Cat # PAB18192).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFATC4 polyclonal antibody (Cat # PAB18192).
Peptide "+" means "peptide blocking".Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFATC4
Entrez GeneID
4776Protein Accession#
Q14934Gene Name
NFATC4
Gene Alias
NF-ATc4, NFAT3
Gene Description
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
Omim ID
602699Gene Ontology
HyperlinkGene Summary
The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq
Other Designations
T cell transcription factor NFAT3|nuclear factor of activated T-cells, cytoplasmic 4
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Interactome
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Pathway
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Disease
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Publication Reference
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M.
Cell 2006 Nov; 127(3):635.
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
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