MITF polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MITF.
Immunogen
A synthetic peptide corresponding to residues surrounding S180/S73 of human MITF.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is specific to MITF.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells (Lane 1) and COLO 205 cells (Lane 2 and 3), using MITF polyclonal antibody (Cat # PAB18189).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skin tissue using MITF polyclonal antibody (Cat # PAB18189).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using MITF polyclonal antibody (Cat # PAB18189).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — MITF
Entrez GeneID
4286Protein Accession#
O75030Gene Name
MITF
Gene Alias
MI, WS2A, bHLHe32
Gene Description
microphthalmia-associated transcription factor
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000195123|OTTHUMP00000195140|homolog of mouse microphthalmia
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Interactome
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Pathway
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Disease
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Publication Reference
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Migration and fate of vestibular melanocytes during development of the human inner ear.
Edward S A van Beelen, Wouter H van der Valk, John C M J de Groot, Erik F Hensen, Heiko Locher, Peter Paul G van Benthem.
Developmental Neurobiology 2020 Nov; 80(11-12):411.
Application:IHC-P, Human, Human embryo, Human otic vesicle.
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Roles of MITF for development of mast cells in mice: effects on both precursors and tissue environments.
Morii E, Oboki K, Ishihara K, Jippo T, Hirano T, Kitamura Y.
Blood 2004 Sep; 104(6):1656.
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The basic helix-loop-helix leucine zipper transcription factor Mitf is conserved in Drosophila and functions in eye development.
Hallsson JH, Haflidadóttir BS, Stivers C, Odenwald W, Arnheiter H, Pignoni F, Steingrímsson E.
Genetics 2004 May; 167(1):233.
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Importance of leucine zipper domain of mi transcription factor (MITF) for differentiation of mast cells demonstrated using mi(ce)/mi(ce) mutant mice of which MITF lacks the zipper domain.
Morii E, Ogihara H, Kim DK, Ito A, Oboki K, Lee YM, Jippo T, Nomura S, Maeyama K, Lamoreux ML, Kitamura Y.
Blood 2001 Apr; 97(7):2038.
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Migration and fate of vestibular melanocytes during development of the human inner ear.
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