RBCK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The RBCK1 polyclonal antibody (Cat # PAB1765) is used in Western blot to detect RBCK1 in mouse kidney tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RBCK1 polyclonal antibody (Cat # PAB1765) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RBCK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RBCK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The RBCK1 polyclonal antibody (Cat # PAB1765) is used in Western blot to detect RBCK1 in mouse kidney tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RBCK1 polyclonal antibody (Cat # PAB1765) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — RBCK1
Entrez GeneID
10616Protein Accession#
NP_112506;Q9BYM8Gene Name
RBCK1
Gene Alias
C20orf18, HOIL1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4
Gene Description
RanBP-type and C3HC4-type zinc finger containing 1
Omim ID
610924Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to mouse UIP28/UbcM4 interacting protein. Alternative splicing has been observed at this locus, resulting in distinct isoforms. [provided by RefSeq
Other Designations
HBV associated factor 4|OTTHUMP00000029921|OTTHUMP00000029922|RBCC protein interacting with PKC1|hepatitis B virus X-associated protein 4|ubiquitin conjugating enzyme 7 interacting protein 3
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Interactome
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Publication Reference
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Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2.
Yamanaka K, Ishikawa H, Megumi Y, Tokunaga F, Kanie M, Rouault TA, Morishima I, Minato N, Ishimori K, Iwai K.
Nature Cell Biology 2003 Apr; 5(4):336.
Application：IP, WB, Human, RD4 cells.
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A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4.
Martinez-Noel G, Niedenthal R, Tamura T, Harbers K.
FEBS Letters 1999 Jul; 454(3):275.
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Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2.
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