ATP5I polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATP5I.
Immunogen
A synthetic peptide corresponding to C-terminus of human ATP5I.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total ATP5I protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using ATP5I polyclonal antibody (Cat # PAB17615).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATP5I
Entrez GeneID
521Protein Accession#
P56385Gene Name
ATP5I
Gene Alias
ATP5K, MGC12532
Gene Description
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E
Omim ID
601519Gene Ontology
HyperlinkGene Summary
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the F0 complex. [provided by RefSeq
Other Designations
ATP synthase e chain, mitochondrial|F1F0-ATP synthase, murine e subunit
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