TAF5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells, using TAF5 polyclonal antibody (Cat # PAB17389).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using TAF5 polyclonal antibody (Cat # PAB17389).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TAF5.
Immunogen
A synthetic peptide corresponding to internal of human TAF5.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total TAF5 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells, using TAF5 polyclonal antibody (Cat # PAB17389).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using TAF5 polyclonal antibody (Cat # PAB17389).
Peptide "+" means "with peptide blocking". -
Gene Info — TAF5
Entrez GeneID
6877Protein Accession#
Q15542Gene Name
TAF5
Gene Alias
TAF2D, TAFII100
Gene Description
TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa
Omim ID
601787Gene Ontology
HyperlinkGene Summary
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. [provided by RefSeq
Other Designations
OTTHUMP00000020402|TATA box binding protein (TBP)-associated factor 2D|TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD|TBP-associated factor 5|transcription initiation factor TFIID 100 kD subunit
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Interactome
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Pathway
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Publication Reference
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Specific interactions and potential functions of human TAFII100.
Tao Y, Guermah M, Martinez E, Oelgeschlager T, Hasegawa S, Takada R, Yamamoto T, Horikoshi M, Roeder RG.
The Journal of Biological Chemistry 1997 Mar; 272(10):6714.
Application：IP, WB-Ce, Human, HeLa cells.
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Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100.
Tanese N, Saluja D, Vassallo MF, Chen JL, Admon A.
PNAS 1996 Nov; 93(24):13611.
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Distinct domains of hTAFII100 are required for functional interaction with transcription factor TFIIF beta (RAP30) and incorporation into the TFIID complex.
Dubrovskaya V, Lavigne AC, Davidson I, Acker J, Staub A, Tora L.
The EMBO Journal 1996 Jul; 15(14):3702.
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Specific interactions and potential functions of human TAFII100.
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