TNF polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using TNF polyclonal antibody (Cat # PAB16934) shows detection of recombinant Swine TNFa at about 16.9 KDa raised in yeast. The identity of the faint band at 135 KDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with 1 ug/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant TNF.
Immunogen
Recombinant protein corresponding to 154 amino acids of swine TNF.
Host
Rabbit
Reactivity
Pig
Form
Lyophilized
Recommend Usage
ELISA (1:2000-1:2500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using TNF polyclonal antibody (Cat # PAB16934) shows detection of recombinant Swine TNFa at about 16.9 KDa raised in yeast. The identity of the faint band at 135 KDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with 1 ug/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Beta-catenin protein utilized by Tumour necrosis factor-alpha in porcine preadipocytes to suppress differentiation.
Luo X, Li HX, Liu RX, Wu ZS, Yang YJ, Yang GS.
BMB Reports 2009 Jun; 42(6):338.
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L-cysteine supplementation attenuates local inflammation and restores gut homeostasis in a porcine model of colitis.
Kim CJ, Kovacs-Nolan J, Yang C, Archbold T, Fan MZ, Mine Y.
Biochimica et Biophysica Acta 2009 Jun; 1790(10):1161.
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Regulation of neutrophil apoptosis by tumor necrosis factor-alpha: requirement for TNFR55 and TNFR75 for induction of apoptosis in vitro.
Murray J, Barbara JA, Dunkley SA, Lopez AF, Van Ostade X, Condliffe AM, Dransfield I, Haslett C, Chilvers ER.
Blood 1997 Oct; 90(7):2772.
Application：Blocking, Human, Human neutrophils.
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Beta-catenin protein utilized by Tumour necrosis factor-alpha in porcine preadipocytes to suppress differentiation.
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