IL33 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using IL33 polyclonal antibody (Cat # PAB16924) shows detection of a band ~18 KDa in size corresponding to recombinant human IL33. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL33.
Immunogen
Recombinant protein corresponding to full length human IL33.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
ELISA (1:20000-1:100000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using IL33 polyclonal antibody (Cat # PAB16924) shows detection of a band ~18 KDa in size corresponding to recombinant human IL33. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL33
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Interactome
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Disease
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Publication Reference
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Mature interleukin-33 is produced by calpain-mediated cleavage in vivo.
Hayakawa M, Hayakawa H, Matsuyama Y, Tamemoto H, Okazaki H, Tominaga S.
Biochemical and Biophysical Research Communications 2009 Jul; 387(1):218.
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Suppression of interleukin-33 bioactivity through proteolysis by apoptotic caspases.
Luthi AU, Cullen SP, McNeela EA, Duriez PJ, Afonina IS, Sheridan C, Brumatti G, Taylor RC, Kersse K, Vandenabeele P, Lavelle EC, Martin SJ.
Immunity 2009 Jun; 31(1):84.
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IL-33, a recently identified interleukin-1 gene family member, is expressed in human adipocytes.
Wood IS, Wang B, Trayhurn P.
Biochemical and Biophysical Research Communications 2009 Apr; 384(1):105.
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Mature interleukin-33 is produced by calpain-mediated cleavage in vivo.
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