IL32 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using IL32 polyclonal antibody (Cat # PAB16923) shows detection of a band ~19 KDa in size corresponding to recombinant human IL32. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant IL32.
Immunogen
Recombinant protein corresponding to full length human IL32.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
ELISA (1:20000-1:100000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot using IL32 polyclonal antibody (Cat # PAB16923) shows detection of a band ~19 KDa in size corresponding to recombinant human IL32. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).Enzyme-linked Immunoabsorbent Assay
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Gene Info — IL32
Entrez GeneID
9235Gene Name
IL32
Gene Alias
IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd
Gene Description
interleukin 32
Omim ID
606001Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
natural killer cell transcript 4|natural killer cells protein 4|tumor necrosis factor alpha-inducing factor
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Interactome
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Publication Reference
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Interleukin-32 expression in the pancreas.
Nishida A, Andoh A, Inatomi O, Fujiyama Y.
The Journal of Biological Chemistry 2009 Apr; 284(26):17868.
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Molecular characterization of IL-32 in human endothelial cells.
Kobayashi H, Lin PC.
Cytokine 2009 Apr; 46(3):351.
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Negative feedback regulation of IL-32 production by iNOS activation in response to dsRNA or influenza virus infection.
Li W, Yang F, Liu Y, Gong R, Liu L, Feng Y, Hu P, Sun W, Hao Q, Kang L, Wu J, Zhu Y.
European Journal of Immunology 2009 Apr; 39(4):1019.
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Interleukin-32 expression in the pancreas.
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