GDF15 polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Western blot using GDF15 polyclonal antibody (Cat # PAB16899) shows detection of a 42 KDa band corresponding to purified recombinant fusion protein MBP-human GDF15. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1 : 1000 in 1% BSA-TBST overnight at 4°C. DyLight649™ Goat Anti-Rabbit IgG secondary antibody was used at 1 : 20,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant GDF15.
Immunogen
Recombinant protein corresponding to human GDF15.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
ELISA (1:500)
Western Blot (1:800-1:1500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using GDF15 polyclonal antibody (Cat # PAB16899) shows detection of a 42 KDa band corresponding to purified recombinant fusion protein MBP-human GDF15. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Primary antibody was used at 1 : 1000 in 1% BSA-TBST overnight at 4°C. DyLight649™ Goat Anti-Rabbit IgG secondary antibody was used at 1 : 20,000 in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GDF15
Entrez GeneID
9518Gene Name
GDF15
Gene Alias
GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Gene Description
growth differentiation factor 15
Omim ID
605312Gene Ontology
HyperlinkGene Summary
Bone morphogenetic proteins (e.g., BMP5; MIM 112265) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM
Other Designations
NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|PTGF-beta|prostate differentiation factor
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Interactome
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Disease
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Publication Reference
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Changes in gene expression contribute to cancer prevention by COX inhibitors.
Baek SJ, Eling TE.
Progress in Lipid Research 2005 Nov; 45(1):1.
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H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.
Lindmark F, Zheng SL, Wiklund F, Bensen J, Balter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Gronberg H, Xu J.
Journal of the National Cancer Institute 2004 Aug; 96(16):1248.
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Changes in gene expression contribute to cancer prevention by COX inhibitors.
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