ACOT13 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ACOT13 in HepG2 cell lysate with ACOT13 polyclonal antibody (Cat # PAB16788) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry
Immunohistochemistry of ACOT13 in human liver tissue with ACOT13 polyclonal antibody (Cat # PAB16788) at 2.5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACOT13.
Immunogen
A synthetic peptide corresponding to C-terminus 17 amino acids of human ACOT13.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ACOT13 in HepG2 cell lysate with ACOT13 polyclonal antibody (Cat # PAB16788) at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunohistochemistry
Immunohistochemistry of ACOT13 in human liver tissue with ACOT13 polyclonal antibody (Cat # PAB16788) at 2.5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — THEM2
Entrez GeneID
55856Protein Accession#
NP_060943Gene Name
THEM2
Gene Alias
HT012, MGC4961, PNAS-27
Gene Description
thioesterase superfamily member 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
15 Kd protein|OTTHUMP00000016090|OTTHUMP00000039398|hypothalamus protein HT012
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Interactome
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Disease
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Publication Reference
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Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs.
Wei J, Kang HW, Cohen DE.
The Biochemical Journal 2009 Jun; 421(2):311.
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Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD2.
Scapa EF, Pocai A, Wu MK, Gutierrez-Juarez R, Glenz L, Kanno K, Li H, Biddinger S, Jelicks LA, Rossetti L, Cohen DE.
FASEB Journal 2008 Jul; 22(7):2579.
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Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule.
Cheng Z, Bao S, Shan X, Xu H, Gong W.
Biochemical and Biophysical Research Communications 2006 Dec; 350(4):850.
Application：IF, WB, Human, HCT-116, U2-OS cells.
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Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs.
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