SWI6 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of crude extract of S. pombe detects a protein with an apparent molecular size of 53 KDa. Using SWI6 polyclonal antibody (Cat # PAB15585).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant SWI6.
Immunogen
Recombinant protein corresponding to full length S. pombe SWI6.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody reacts with SWI6 of S. pombe. Although genome data indicate the swi6 gene encodes a protein of 37 KDa, Western blot analysis of crude extract of S. pombe detects a protein with an apparent molecular size of 53 KDa.
Form
Liquid
Recommend Usage
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.09% sodium azide)..
Storage Instruction
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of crude extract of S. pombe detects a protein with an apparent molecular size of 53 KDa. Using SWI6 polyclonal antibody (Cat # PAB15585).Immunoprecipitation
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Gene Info — SWI6
Entrez GeneID
850879Protein Accession#
P40381Gene Name
SWI6
Gene Alias
PSL8, SDS11
Gene Description
Swi6p
Gene Ontology
HyperlinkGene Summary
forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate
Other Designations
Transcription cofactor, forms complexes with DNA-binding proteins Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; localization regulated by phosphorylation; potential Cdc28p substrate
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Publication Reference
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Ers1 links HP1 to RNAi.
Rougemaille M, Braun S, Coyle S, Dumesic PA, Garcia JF, Isaac RS, Libri D, Narlikar GJ, Madhani HD.
PNAS 2012 Jul; 109(28):11258.
Application：WB, Yeast, Yeast.
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Programmed DNA deletion as an RNA-guided system of genome defense.
Yao MC, Fuller P, Xi X.
Science 2003 Jun; 300(5625):1581.
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Conservation of heterochromatin protein 1 function.
Wang G, Ma A, Chow CM, Horsley D, Brown NR, Cowell IG, Singh PB.
Molecular and Cellular Biology 2000 Sep; 20(18):6970.
Application：WB, Yeast, Yeast cells.
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The chromodomain protein Swi6: a key component at fission yeast centromeres.
Ekwall K, Javerzat JP, Lorentz A, Schmidt H, Cranston G, Allshire R.
Science 1995 Sep; 269(5229):1429.
Application：IF, WB-Ce, WB-Tr, Yeast, Yeast cells.
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Ers1 links HP1 to RNAi.
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