SUP35 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SUP35.
Immunogen
A synthetic peptide corresponding to amino acids 494-507 of Saccharomyces cerevisiae SUP35.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody reacts with S. cerevisiae SUP35.
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Identification of SUP35 protein in crude extracts of S. cerevisiae by western blotting with SUP35 polyclonal antibody (Cat # PAB15578) was used at 1/2,000 dilution. SUP35 protein was identified about 77 kDa position (Molecular mass of SUP35 is 76.6 kDa).
Lane 1: Endogenous expression in S. cerevisiae.
Lane 2: Overexpression of SUP35 protein by introduction of a plasmid carrying SUP35 gene into S. cerevisiae. -
Gene Info — SUP35
Entrez GeneID
851752Gene Name
SUP35
Gene Alias
GST1, PNM2, SAL3, SUF12, SUP2, SUP36
Gene Description
Sup35p
Gene Ontology
HyperlinkGene Summary
a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype
Other Designations
Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype
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Publication Reference
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Targeted identification of protein interactions in eukaryotic mRNA translation.
Link AJ, Niu X, Weaver CM, Jennings JL, Duncan DT, McAfee KJ, Sammons M, Gerbasi VR, Farley AR, Fleischer TC, Browne CM, Samir P, Galassie A, Boone B.
Proteomics 2020 Apr; 20(7):e1900177.
Application:WB, Yeast, S. cerevisiae TAP strain.
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The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions.
Kimura Y, Koitabashi S, Kakizuka A, Fujita T.
Genes to Cells: Devoted to Molecular & Cellular Mechanisms 2004 Aug; 9(8):685.
Application:WB, Yeast , Yeast extracts.
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Propagation of the yeast prion-like [psi+] determinant is mediated by oligomerization of the SUP35-encoded polypeptide chain release factor.
Paushkin SV, Kushnirov VV, Smirnov VN, Ter-Avanesyan MD.
The EMBO Journal 1996 Jun; 15(12):3127.
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Role of the chaperone protein Hsp104 in propagation of the yeast prion-like factor [psi+].
Chernoff YO, Lindquist SL, Ono B, Inge-Vechtomov SG, Liebman SW.
Science 1995 May; 268(5212):880.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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Targeted identification of protein interactions in eukaryotic mRNA translation.
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