RAD18 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Identification of RAD18 protein in crude extract of A-549 cells by western blotting with RAD18 polyclonal antibody (Cat # PAB15575). The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.
Immunofluorescence
Indirect immunofluorescence staining of RAD18 protein in GM637 cells by RAD18 polyclonal antibody (Cat # PAB15575). RAD18 protein is stained as yellow dots in nuclei.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant RAD18.
Immunogen
Recombinant GST fusion protein corresponding to C-terminus 113 amino acids of human RAD18.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to human RAD18.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Identification of RAD18 protein in crude extract of A-549 cells by western blotting with RAD18 polyclonal antibody (Cat # PAB15575). The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.Immunofluorescence
Indirect immunofluorescence staining of RAD18 protein in GM637 cells by RAD18 polyclonal antibody (Cat # PAB15575). RAD18 protein is stained as yellow dots in nuclei.Immunoprecipitation
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Gene Info — RAD18
Entrez GeneID
56852Gene Name
RAD18
Gene Alias
RNF73
Gene Description
RAD18 homolog (S. cerevisiae)
Omim ID
605256Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq
Other Designations
RAD18, S. cerevisiae, homolog|postreplication repair protein hRAD18p
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Interactome
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Disease
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Publication Reference
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Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination.
Miyase S, Tateishi S, Watanabe K, Tomita K, Suzuki K, Inoue H, Yamaizumi M.
The Journal of Biological Chemistry 2005 Jan; 280(1):515.
Application：IF, WB-Tr, Monkey, Mouse , COS-7, GM637 cells, Mouse fibroblasts.
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Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination.
Watanabe K, Tateishi S, Kawasuji M, Tsurimoto T, Inoue H, Yamaizumi M.
The EMBO Journal 2004 Oct; 23(19):3886.
Application：IF, IP, WB, Human, Mouse, Cell lines from RAD18 knockout mice, GM637 cells.
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Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.
Tateishi S, Sakuraba Y, Masuyama S, Inoue H, Yamaizumi M.
PNAS 2000 Jul; 97(14):7927.
Application：ICC, IF, Human, Human skin fibroblasts.
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Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination.
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