Histone H3 (K4me2) polyclonal antibody
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More Files
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ChIP
Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) was used in ChIP with murine embryonic stem cells (Lane 1) or erythroleukemia cells (Lane 2).
Top Panel : PCR primers specifc for beta-globulin were used to amplify a 210 base pair promoter following DNA isolation.
Lower Panel : Input DNA control.
Western Blot (Cell lysate)
Acid treated HeLa (10 ug) extract was treated with a 1 : 500 dilution of antibody Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) prior to visualization.
Dot Blot (Peptide)
To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the Lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
Lanes 9-12 contained peptides encompassing the residue lysine 27 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me2).
Immunogen
Lysine4 dimethylated peptide of Histone H3.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody is expected to react with Histone H3 from yeast to mammals.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) was used in ChIP with murine embryonic stem cells (Lane 1) or erythroleukemia cells (Lane 2).
Top Panel : PCR primers specifc for beta-globulin were used to amplify a 210 base pair promoter following DNA isolation.
Lower Panel : Input DNA control.Western Blot (Cell lysate)
Acid treated HeLa (10 ug) extract was treated with a 1 : 500 dilution of antibody Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) prior to visualization.Dot Blot (Peptide)
To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the Lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
Lanes 9-12 contained peptides encompassing the residue lysine 27 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
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