Histone H3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3.
Immunogen
A synthetic peptide corresponding to C-terminus of Histone H3.
Host
Rabbit
Reactivity
Yeast
Specificity
This antibody is expected to react with Histone H3 from yeast to mammals.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1500)
ChIP (50 ul)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
Western Blot
5 uL of acid treated HeLa extracts or recombinant Histone H3 were treated with a 1 : 500 dilution of Histon H3 polyclonal antibody (Cat # PAB15451) prior to visualization.
Lane 1 : HeLa Extract.
Lane 2 : HeLa cells treated with sodium butyrate.
Lane 3 : Recombinant human Histone H3 (0.2 ug).Immunoprecipitation
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