Beta-Galactosidase polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against beta-Galactosidase.
Immunogen
Recombinant protein corresponding to full length Escherichia coli beta-Galactosidase.
Host
Goat
Specificity
It shows reactivity to mammalian beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of E. coli.
Form
Liquid
Preparation Method
The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02 M sodium phosphate, 0.14 M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 um filter, and vialed. No preservative is added.
Isotype
IgG
Recommend Usage
ELISA (1:400-1:2000)
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM sodium phosphate buffer, 140 mM NaCl, pH 7.3.
Storage Instruction
Store at 4°C.
Note
Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
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Applications
Western Blot
Immunohistochemistry
Enzyme Immunoassay
Immunodiffusion
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