SLIRP polyclonal antibody (DyLight 650)
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SLIRP.
Immunogen
A synthetic peptide corresponding to C-terminus of human SLIRP.
Host
Rabbit
Reactivity
Human, Primates
Specificity
This antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen ~12 kDa representing the SLIRP protein. May see additional bands above 45 kDa. In ICC/IF, staining of the mitochondria was observed in U2OS cells. In IHC-P, cytoplasmic and mitochondrial staining was observed in mouse intestine tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Form
Liquid
Conjugation
DyLight 650
Purification
Immunogen affinity purification
Recommend Usage
Immunocytochemistry/Immunofluorescence
Immunohistochemistry
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM sodium borate (0.05% sodium azide).
Storage Instruction
Store at 4°C in the dark.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
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Gene Info — SLIRP
Entrez GeneID
81892Protein Accession#
Q9GZT3Gene Name
SLIRP
Gene Alias
DC23, C14orf156, DC50, PD04872
Gene Description
SRA stem-loop interacting RNA binding protein
Omim ID
610211Gene Ontology
HyperlinkGene Summary
Steroid receptor RNA activator (SRA, or SRA1; MIM 603819) is a complex RNA molecule containing multiple stable stem-loop structures that functions in coactivation of nuclear receptors. SLIRP interacts with stem-loop structure-7 of SRA (STR7) and modulates nuclear receptor transactivation (Hatchell et al., 2006 [PubMed 16762838]).[supplied by OMIM
Other Designations
SRA stem-loop-interacting RNA-binding protein, mitochondrial
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