EGLN1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis of human EGLN1, using EGLN1 polyclonal antibody (Cat # PAB14555).
Lane 1 : Recombinant FLAG-His-PHD1 (10ng/lane).
Lane 2 : PHD2 (10ng/lane) .
Lane 3 : PHD3 (10ng/lane).
Lane 4 : Whole cell lysate from HeLa cells.
Lane 5 : Hela with hypoxia treatment.
Immunohistochemistry
Staining of Vascular Endothelium 40X in human lung using EGLN1 polyclonal antibody (Cat # PAB14555).
Flow Cytometry
Flow cytometric detection of EGLN1. 106 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/mL EGLN1 polyclonal antibody (Cat # PAB14555) in a 150 uL reaction.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGLN1.
Immunogen
A synthetic peptide corresponding to amino acids 1-50 of human EGLN1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to human HIF prolyl hydroxylase 2.
Form
Liquid
Recommend Usage
Flow Cytometry (3 mg/mL)
Western Blot (1:500-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-citrate/phosphate buffer, pH 7-8 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human EGLN1, using EGLN1 polyclonal antibody (Cat # PAB14555).
Lane 1 : Recombinant FLAG-His-PHD1 (10ng/lane).
Lane 2 : PHD2 (10ng/lane) .
Lane 3 : PHD3 (10ng/lane).
Lane 4 : Whole cell lysate from HeLa cells.
Lane 5 : Hela with hypoxia treatment.Immunohistochemistry
Staining of Vascular Endothelium 40X in human lung using EGLN1 polyclonal antibody (Cat # PAB14555).Flow Cytometry
Flow cytometric detection of EGLN1. 106 Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/mL EGLN1 polyclonal antibody (Cat # PAB14555) in a 150 uL reaction. -
Gene Info — EGLN1
Entrez GeneID
54583Protein Accession#
NP_071334.1Gene Name
EGLN1
Gene Alias
C1orf12, DKFZp761F179, ECYT3, HIFPH2, HPH2, PHD2, SM-20, SM20, ZMYND6
Gene Description
egl nine homolog 1 (C. elegans)
Gene Ontology
HyperlinkOther Designations
HIF prolyl hydroxylase 2|egl nine homolog 1|egl nine-like protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Concordant regulation of gene expression by hypoxia and 2-oxoglutarate-dependent dioxygenase inhibition: the role of HIF-1alpha, HIF-2alpha, and other pathways.
Elvidge GP, Glenny L, Appelhoff RJ, Ratcliffe PJ, Ragoussis J, Gleadle JM.
The Journal of Biological Chemistry 2006 Jun; 281(22):15215.
Application：WB-Tr, Human, MCF-7 cells.
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Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness.
Jokilehto T, Rantanen K, Luukkaa M, Heikkinen P, Grenman R, Minn H, Kronqvist P, Jaakkola PM.
Clinical Cancer Research 2006 Feb; 12(4):1080.
Application：IF, IHC, WB-Ce, WB-Tr, Human, Head, neck squamous cell carcinoma, HeLa, Fibroblasts, HUVEC, HaCaT, UT-SCC8, UT-SCC9 cells.
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Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW.
PNAS 2005 Jul; 102(29):10212.
Application：WB, Human, HLMVE-A cells.
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Concordant regulation of gene expression by hypoxia and 2-oxoglutarate-dependent dioxygenase inhibition: the role of HIF-1alpha, HIF-2alpha, and other pathways.
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