NKX2-5 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NKX2-5 polyclonal antibody (Cat # PAB14394) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NKX2-5 polyclonal antibody (Cat # PAB14394) (4 ug/mL) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence
NKX2-5 polyclonal antibody (Cat # PAB14394) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NKX2-5.
Immunogen
A synthetic peptide corresponding to human NKX2-5.
Sequence
C-PRAYSDPDPAKDPR
Host
Goat
Theoretical MW (kDa)
34.9, 11.7, 16.1
Reactivity
Human, Mouse, Rat
Specificity
Approx 45 KDa band observed in human heart lysates (calculated MW of 34.9 KDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (4 ug/mL)
Immunofluorescence (10 ug/mL)
Western Blot (0.1-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NKX2-5 polyclonal antibody (Cat # PAB14394) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NKX2-5 polyclonal antibody (Cat # PAB14394) (4 ug/mL) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Immunofluorescence
NKX2-5 polyclonal antibody (Cat # PAB14394) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NKX2-5
Entrez GeneID
1482Protein Accession#
NP_004378.1;NP_001159647.1;NP_001159648.1Gene Name
NKX2-5
Gene Alias
CHNG5, CSX, CSX1, NKX2.5, NKX2E, NKX4-1
Gene Description
NK2 transcription factor related, locus 5 (Drosophila)
Gene Ontology
HyperlinkGene Summary
Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM
Other Designations
NK2 transcription factor homolog E|NK2 transcription factor related, locus 5|cardiac-specific homeo box|tinman paralog
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Interactome
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Disease
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Publication Reference
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Abcc6 Deficiency Causes Increased Infarct Size and Apoptosis in a Mouse Cardiac Ischemia-Reperfusion Model.
Mungrue IN, Zhao P, Yao Y, Meng H, Rau C, Havel JV, Gorgels TG, Bergen AA, Maclellan WR, Drake TA, Bostrom KI, Lusis AJ.
Arteriosclerosis, Thrombosis, and Vascular Biology 2011 Dec; 31(12):2806.
Application：WB-Ti, Mouse, Mouse hearts.
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Perinatal loss of Nkx2-5 results in rapid conduction and contraction defects.
Briggs LE, Takeda M, Cuadra AE, Wakimoto H, Marks MH, Walker AJ, Seki T, Oh SP, Lu JT, Sumners C, Raizada MK, Horikoshi N, Weinberg EO, Yasui K, Ikeda Y, Chien KR, Kasahara H.
Circulation Research 2008 Sep; 103(6):580.
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Abcc6 Deficiency Causes Increased Infarct Size and Apoptosis in a Mouse Cardiac Ischemia-Reperfusion Model.
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