H3K27me2 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K27me2 polyclonal antibody (Cat # PAB14116), crude serum and flow through.
The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 16,900.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of H3K27me2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the dimethylated lysine 27 (H3K27me2).
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:500)
Dot Blot (1:20000)
Western Blot (1:1000)
ChIP (5 ug/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
ChIP
Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of H3K27me2 polyclonal antibody (Cat # PAB14116), crude serum and flow through.
The antigen used was a peptide containing the histone modification of interest.
By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 16,900.Dot Blot
-
Publication Reference
-
Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.
Molecular Cell 2007 Jan; 25(1):1.
-
Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.
The Journal of Biological Chemistry 2003 Apr; 278(26):24217.
-
Translating the histone code.
Jenuwein T, Allis CD.
Science 2001 Aug; 293(5532):1074.
-
Dynamic regulation of histone lysine methylation by demethylases.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com