COL2A1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against native COL2A1.
Immunogen
Native purified COL2A1 from bovine cartilage.
Host
Rabbit
Reactivity
Bovine, Horse, Pig, Sheep
Specificity
This antibody reacts with bovine collagen type II 100%, bovine collagen XI <3.0 %; bovine collagen I and IX <0.1% (RIA at 1:200 dilution).
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:100-1:500)
Immunofluorescence (1:40)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS buffer (without BSA and preservative).
Storage Instruction
Store at -20°C.
After reconstitution with 100 uL of deionized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
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Gene Info — COL2A1
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Publication Reference
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A biomimetic honeycomb-like scaffold prepared by flow-focusing technology for cartilage regeneration.
Wang CC, Yang KC, Lin KH, Wu CC, Liu YL, Lin FH, Chen IH.
Biotechnology and Bioengineering 2014 Nov; 111(11):2338.
Application：IHC, Porcine, Chondrocytes.
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Cartilage regeneration in SCID mice using a highly organized three-dimensional alginate scaffold.
Wang CC, Yang KC, Lin KH, Liu YL, Liu HC, Lin FH.
Biomaterials 2012 Jan; 33(1):120.
Application：IHC-P, Mouse, Chondrocyte/scaffold constructs.
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A biomimetic honeycomb-like scaffold prepared by flow-focusing technology for cartilage regeneration.
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