LASS5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LASS5.
Immunogen
A synthetic peptide corresponding to C-terminus 17 amino acids of human LASS5.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LASS5 in SK-N-SH lysate with LASS5 polyclonal antibody (Cat # PAB13439) at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.Immunofluorescence
Immunofluorescence staining of human brain tissue with 20 ug/mL LASS5 polyclonal antibody (Cat # PAB13439). -
Gene Info — LASS5
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Interactome
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Publication Reference
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Necessary role for the Lag1p motif in (dihydro)ceramide synthase activity.
Spassieva S, Seo JG, Jiang JC, Bielawski J, Alvarez-Vasquez F, Jazwinski SM, Hannun YA, Obeid LM.
The Journal of Biological Chemistry 2006 Nov; 281(45):33931.
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LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor.
Lahiri S, Futerman AH.
The Journal of Biological Chemistry 2005 Aug; 280(40):33735.
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Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donors.
Riebeling C, Allegood JC, Wang E, Merrill AH Jr, Futerman AH.
The Journal of Biological Chemistry 2003 Oct; 278(44):43452.
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Necessary role for the Lag1p motif in (dihydro)ceramide synthase activity.
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