TP53INP1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TP53INP1.
Immunogen
A synthetic peptide corresponding to C-terminus 14 amino acids of human TP53INP1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This sequence is identical between a and b forms of the PUMA proteins. A band at 27 KDa can be detected. A lower band at 18 KDa was detected in human spleen, and mouse liver and kidney tissue lysates, which may represent the p53DINP1-b form. It is human, mouse, and rat reactive.
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of TP53INP1 expression in human lung tissue lysate with TP53INP1 polyclonal antibody (Cat # PAB12887) at 2 ug/mL in the absence (lane 1) or presence (lane 2) of blocking peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of mouse liver using TP53INP1 polyclonal antibody (Cat # PAB12887) at 2 ug/mL . -
Gene Info — TP53INP1
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Interactome
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Publication Reference
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Molecular and functional characterization of the stress-induced protein (SIP) gene and its two transcripts generated by alternative splicing. SIP induced by stress and promotes cell death.
Tomasini R, Samir AA, Vaccaro MI, Pebusque MJ, Dagorn JC, Iovanna JL, Dusetti NJ.
The Journal of Biological Chemistry 2001 Nov; 276(47):44185.
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p53DINP1, a p53-inducible gene, regulates p53-dependent apoptosis.
Okamura S, Arakawa H, Tanaka T, Nakanishi H, Ng CC, Taya Y, Monden M, Nakamura Y.
Molecular Cell 2001 Jul; 8(1):85.
Application:IF, WB-Ce, Human, MCF7 cells.
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Molecular and functional characterization of the stress-induced protein (SIP) gene and its two transcripts generated by alternative splicing. SIP induced by stress and promotes cell death.
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