SCGB2A2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from MCF-7 was immuno-blotted by SCGB2A1 polyclonal antibody (Cat # PAB12703) at 1 : 500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue stained with SCGB2A1 polyclonal antibody (Cat # PAB12703) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SCGB2A2.
Immunogen
A synthetic peptide corresponding to internal region of human SCGB2A2.
Host
Rabbit
Theoretical MW (kDa)
10
Reactivity
Human
Specificity
This antibody only recognizes ~10 KDa of human SCGB2A2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from MCF-7 was immuno-blotted by SCGB2A1 polyclonal antibody (Cat # PAB12703) at 1 : 500.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast cancer tissue stained with SCGB2A1 polyclonal antibody (Cat # PAB12703) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Mammaglobin B gene as a novel marker for lymph node micrometastasis in patients with abdominal cancers.
Aihara T, Fujiwara Y, Miyake Y, Okami J, Okada Y, Iwao K, Sugita Y, Tomita N, Sakon M, Shiozaki H, Monden M.
Cancer Letters 2000 Mar; 150(1):79.
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Mammaglobin B gene as a novel marker for lymph node micrometastasis in patients with abdominal cancers.
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