IRS1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IRS1.
Immunogen
A synthetic peptide corresponding to the epitope ATSPA without phosphorylation of human IRS1.
Host
Rabbit
Theoretical MW (kDa)
165
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~165 KDa of human IRS1 (without phosphorylation at Ser312).
Form
Liquid
Purification
Site-specific epitope affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (Antibody stabilizer).
Storage Instruction
Store at 4°C for three month without detectable loss of activity. For long term storage, store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 polyclonal antibody (Cat # PAB12695), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control.Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane A : IRS1 (pS312).
B : IRS1 (non phosphorylated).
C : Non-related Phosphopeptide.
Followed by rabbit antibodies incubation at a 1 : 1000 dilution.
1. Phospho-IRS1 S312 polyclonal antibody (Cat # PAB12627).
2 : IRS1 polyclonal antibody (Cat # PAB12695). -
Gene Info — IRS1
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD.
The Journal of Biological Chemistry 2004 Sep; 279(44):45304.
Application:IP-WB, Mouse, 3T3-L1, C2C12 cells.
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Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
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