GLUL polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of GLUL using GLUL polyclonal antibody (Cat # PAB12567). 40 ug of lysates from mouse (Lane M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Localization of glutamine synthase in the retina. Human retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase. Nuclei were stained with nuclear fast red. On inspection at low magnification, GLUL polyclonal antibody (Cat # PAB12567) reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL).
Immunofluorescence
Localization of glutamine synthase in the retina.
Paraffin sections of mouse (A, B), rat (C, D, G-I) were reacted with GLUL polyclonal antibody (Cat # PAB12567).
Staining red fluorescence in B, D, G, I, and brown immuno-peroxidase reaction.
Nuclei in some immunofluorescence experiments (A-D) were stained with DAPI (shown in cyan).
On inspection at low magnification, GLUL polyclonal antibody (Cat # PAB12567) reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL).
No signal was detected in controls either pre-incubated with 100 ug/mL of the immunizing peptide (A) or with pre-immune serum (C).
The pattern of staining observed in all experiments is typical of Muller cells.
This finding was confirmed by co-localization (indicated by yellow in I) of GLUL (red in G) with antoher marker of GLUL (green in H).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant GLUL.
Immunogen
Recombinant protein corresponding to GLUL.
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunohistochemistry (1:25000)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of GLUL using GLUL polyclonal antibody (Cat # PAB12567). 40 ug of lysates from mouse (Lane M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Localization of glutamine synthase in the retina. Human retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase. Nuclei were stained with nuclear fast red. On inspection at low magnification, GLUL polyclonal antibody (Cat # PAB12567) reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL).Immunofluorescence
Localization of glutamine synthase in the retina.
Paraffin sections of mouse (A, B), rat (C, D, G-I) were reacted with GLUL polyclonal antibody (Cat # PAB12567).
Staining red fluorescence in B, D, G, I, and brown immuno-peroxidase reaction.
Nuclei in some immunofluorescence experiments (A-D) were stained with DAPI (shown in cyan).
On inspection at low magnification, GLUL polyclonal antibody (Cat # PAB12567) reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL).
No signal was detected in controls either pre-incubated with 100 ug/mL of the immunizing peptide (A) or with pre-immune serum (C).
The pattern of staining observed in all experiments is typical of Muller cells.
This finding was confirmed by co-localization (indicated by yellow in I) of GLUL (red in G) with antoher marker of GLUL (green in H).
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