MARCKS polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of MARCKS in whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with MARCKS polyclonal antibody (Cat # PAB12564) at dilution of 1 : 10,000.
Immunofluorescence
View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and MARCKS polyclonal antibody (Cat # PAB12564), our antibody to MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant MARCKS.
Immunogen
Recombinant protein corresponding to full length MARCKS.
Host
Rabbit
Reactivity
Rat
Specificity
The MARCKS protein runs at about 80KDa on SDS-PAGE gels, although the real molecular weight is much lower, at about 31.5KDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunocytochemistry (1:5000)
Immunofluorescence (1:500-1:1000)
Western Blot (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of MARCKS in whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with MARCKS polyclonal antibody (Cat # PAB12564) at dilution of 1 : 10,000.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
View of mixed neuron/glial cultures stained with our MARCKS antibody (red) and MARCKS polyclonal antibody (Cat # PAB12564), our antibody to MAP2 made in chicken. Note that the MARCKS antibody stains vesicular structures both in the glial cells and in the dendrites of the neurons, which are strongly stained with the MAP2 antibody. -
Publication Reference
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MARCKS deficiency in mice leads to abnormal brain development and perinatal death.
Stumpo DJ, Bock CB, Tuttle JS, Blackshear PJ.
PNAS 1995 Feb; 92(4):944.
Application：WB-Ti, Mouse, Mouse brains.
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The MARCKS family of cellular protein kinase C substrates.
Blackshear PJ.
The Journal of Biological Chemistry 1993 Jan; 268(3):1501.
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Purification of two distinct proteins of approximate Mr 80,000 from human epithelial cells and identification as proper substrates for protein kinase C.
Hirai M, Shimizu N.
The Biochemical Journal 1990 Sep; 270(3):583.
Application：AFC, IP, Rat, Rat purified proteins.
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MARCKS deficiency in mice leads to abnormal brain development and perinatal death.
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