SEPT2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SEPT2.
Immunogen
A synthetic peptide corresponding to human SEPT2.
Sequence
SKQQPTQFINPET-C
Host
Goat
Theoretical MW (kDa)
41.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (2.5 ug/mL)
Western Blot (0.05-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SEPT2 polyclonal antibody (Cat # PAB11438) (0.05 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT2 polyclonal antibody (Cat # PAB11438) (2.5 ug/mL) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT2 polyclonal antibody (Cat # PAB11438) (2.5 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SEPT2 polyclonal antibody (Cat # PAB11438) (2.5 ug/mL) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SEPT2
Entrez GeneID
4735Protein Accession#
NP_001008491.1Gene Name
SEPT2
Gene Alias
DIFF6, KIAA0158, NEDD5, Pnutl3, hNedd5
Gene Description
septin 2
Omim ID
601506Gene Ontology
HyperlinkGene Summary
developmentally down-regulated 5
Other Designations
OTTHUMP00000164575|neural precursor cell expressed, developmentally down-regulated 5
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Interactome
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Disease
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Publication Reference
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Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7.
Kremer BE, Adang LA, Macara IG.
Cell 2007 Sep; 130(5):837.
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Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7.
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