CHK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using CHK1 polyclonal antibody (Cat # PAB11360) shows detection of bands corresponding to CHK1 in Saccharomyces cerevisiae lysates.
Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 KDa. Lane 2 shows a predominant band at ~38 KDa.
Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively).
Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 minat room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CHK1.
Immunogen
A synthetic peptide corresponding to amino acids 312-327 of Saccharomyces cerevisiae CHK1.
Host
Rabbit
Reactivity
Yeast
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using CHK1 polyclonal antibody (Cat # PAB11360) shows detection of bands corresponding to CHK1 in Saccharomyces cerevisiae lysates.
Two strains of S.cerevisiae were tested. Lane 1 shows a predominant band at ~60 KDa. Lane 2 shows a predominant band at ~38 KDa.
Specific band staining is blocked when antibody is preincubated for 45 min at room temperature with 50 ug of peptide immunogen (lanes 3 and 4 respectively).
Lysates were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:750 in blocking buffer diluted 1:5 in PBS.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 minat room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHK1
Entrez GeneID
852577Protein Accession#
P38147;NP_009833Gene Name
CHK1
Gene Alias
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Gene Description
Chk1p
Gene Ontology
HyperlinkGene Summary
mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
Other Designations
Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
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Publication Reference
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Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms.
Sanchez Y, Bachant J, Wang H, Hu F, Liu D, Tetzlaff M, Elledge SJ.
Science 1999 Nov; 286(5442):1166.
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Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms.
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