VDAC1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using VDAC1 polyclonal antibody (Cat # PAB11290) detects a single band at ~32 KDa corresponding to VDAC1 (arrowhead) present in a rat heart tissue lysate.
Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight.
Washes were followed by reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VDAC1.
Immunogen
A synthetic peptide corresponding to amino acids 185-197 of human VDAC1.
Host
Rabbit
Reactivity
Birds, Human
Specificity
This affinity purified antibody is directed against human VDAC1/Porin1 protein. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms).
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using VDAC1 polyclonal antibody (Cat # PAB11290) detects a single band at ~32 KDa corresponding to VDAC1 (arrowhead) present in a rat heart tissue lysate.
Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight.
Washes were followed by reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VDAC1
Entrez GeneID
7416Protein Accession#
P21796(VDAC1);P45880(VDAC2)(isoform1);Q9Y277(VDAC3)(isoform1)Gene Name
VDAC1
Gene Alias
MGC111064, PORIN, PORIN-31-HL
Gene Description
voltage-dependent anion channel 1
Omim ID
604492Gene Ontology
HyperlinkOther Designations
OTTHUMP00000165946|OTTHUMP00000165947
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Interactome
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Pathway
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Disease
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Publication Reference
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Human SCO1 and SCO2 have independent, cooperative functions in copper delivery to cytochrome c oxidase.
Leary SC, Kaufman BA, Pellecchia G, Guercin GH, Mattman A, Jaksch M, Shoubridge EA.
Human Molecular Genetics 2004 Sep; 13(17):1839.
Application：WB, Human, Fibroblasts, Myoblasts.
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Rapid induction of mitochondrial events and caspase-independent apoptosis in Survivin-targeted melanoma cells.
Liu T, Brouha B, Grossman D.
Oncogene 2004 Jan; 23(1):39.
Application：WB-Tr, Human, YUSAC2 cells.
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In vivo adenoviral transfer of sorcin reverses cardiac contractile abnormalities of diabetic cardiomyopathy.
Suarez J, Belke DD, Gloss B, Dieterle T, McDonough PM, Kim YK, Brunton LL, Dillmann WH.
American Journal of Physiology. Heart and Circulatory Physiology 2004 Jan; 286(1):H68.
Application：WB-Ti, Rat, Rat cardiac myocytes.
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Human SCO1 and SCO2 have independent, cooperative functions in copper delivery to cytochrome c oxidase.
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