Rabbit Anti-Pig IgG (H&L) secondary antibody (Alkaline Phosphatase)
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Specification
Product Description
Rabbit polyclonal antibody raised against swine IgG whole molecule. This secondary antibody generated in rabbit detects swine Immunoglobulin G. Both the heavy and light chains of the antibody molecule are present. The antibody is conjugated with alkaline phosphatase.
Immunogen
Swine IgG whole molecule.
Host
Rabbit
Reactivity
Pig
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, swine IgG, and swine serum.
Form
Liquid
Conjugation
Alkaline Phosphatase (Calf Intestine)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Recommend Usage
ELISA (1:14000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.05 M tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, pH 8.0 (50% (v/v) glycerol, 10 mg/mL BSA - immunoglobulin and protease free, 0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C before opening. Do not Freeze. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
Dot Blot
Enzyme Immunoassay
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Publication Reference
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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
Avrameas S, Ternynck T.
Immunochemistry 1971 Dec; 8(12):1175.
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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
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