Cy3/Cy5 polyclonal antibody
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Specification
Product Description
Sheep polyclonal antibody raised against Cy3/Cy5.
Immunogen
A mixture of Cy3 and Cy5 conjugated with KLH.
Host
Sheep
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Form
Liquid
Purification
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Cy3/Cy5.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C prior to opening. For long term storage, store at -20°C or below.
Aliquot to avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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